Collecting And Storing Pollen : Cannabis Males & Reversed Females

Although making seeds is easier than breeding, collecting and storing pollen is an essential skill that all aspiring breeders must master. Whether you are a future cannabis breeder, a hobby pollen chucker or you’re just reading this for “educational purposes”, welcome!
In this tutorial, we are going to go over how to successfully harvest and store pollen from both cannabis males and reversed females.

If you want to do this seriously and create your own stable cannabis strain, it is paramount to learn the basics of cannabis breeding. However you may also want to make seeds just for fun which is fine too. Let’s delve into it!

Collecting Male Pollen

Collecting and storing pollen from a male plant is often easier than harvesting feminized pollen.
Male cannabis plants can produce copious amounts of pollen. Furthermore, a little bit of pollen goes a long way so you will just need a small amount of it to make hundreds of seeds!
The best time to collect male pollen is usually about 1 week after the first flowers begin to open. Keep in mind that this is strain specific so it can vary greatly.

What you need to get started:

  • dehumidifier (Not needed if the Relative Humidity is under 45%)
  • table with a flat surface
  • pair of tweezers
  • sieve or mesh screen
  • plastic card
  • sheet of paper
  • test tubes
  • garden sprayer

Because humidity destroys pollen viability by causing the pollen spores to germinate, the environment has to be very dry. If the Relative Humidity is over 45%, it will be paramount to use a dehumidifier.

Pollen grains are invisible so make sure that you are collecting your pollen in an airtight room or a room far away from your flowering females.

4 Steps To Collecting Pollen

Step 1: Place the male plant above the table with a sieve between the table and the plant. Male cannabis flowers are very moist and this can ruin the viability of your pollen.

Step 2: Gently shake the male clusters above the sieve. The pollen should end up on the table and the male flowers should remain trapped in the sieve. Remove all foliage remnants and male flowers from the pollen powder using your tweezers.

Cannabis Pollen Collection

Step 3: Let the pollen dry with the dehumidifier running for at least 12 hours.
Pollen doesn’t store well at room temperature so the dehumidifier will help suck up the moisture faster.

Step 4: Collect the pollen from the table using a plastic card. Slowly push it onto a sheet of paper previously folded in the middle.

Beware! Always thoroughly spray yourself with water as soon as you leave the room. Pollen grains are tiny and after collecting pollen, your clothes will be full of invisible pollen spores. It is very easy to contaminate your female plants that way.

Short Term Storage VS Long Term Storage

Short term storage:

After waiting for 12+ hours, gently poor the pollen into your airtight test tubes and store them in a fridge.
This pollen should remain viable for 3 to 6+ weeks that way. This method is great whenever growers want to stagger the pollination of their female plants.

Long term storage:

Use an airtight glass jar with a couple of silica gel packs in it. Poor the pollen into a small paper pouch and place the paper pouch into the glass jar. After about 48 hours, you can then mix up the pollen with dry cooking flour (1 part pollen / 1 part flour) and fill up your test tubes.
Finally, the test vials can go straight to the freezer.
Always let the tubes come near room temperature before opening them so as not to draw warm moist air. When done properly, you can store pollen for several years using this method.

Collecting And Storing Pollen From Reversed Females

Harvesting pollen from reversed females is a similar process though it is often trickier. Not all female cannabis plants can be reversed. Some will produce male flowers devoid of pollen, some will produce male flowers with tiny amounts of feminized pollen and some will give male flowers with good amounts of pollen.
Reversed females almost never produce as much pollen as male plants.
For that reason, instead of shaking the clusters above a sieve, it is better to pluck the mature male flowers one by one and tap them against the table using tweezers. By doing that, the small amount of pollen trapped in the flowers should fall off onto the table.
You can use a black table in order to see how much pollen these reversed flowers contain.

Note that reversed females take longer than male plants to start producing pollen. Therefore, feminized seed makers should start reversing their donor females at least 1 week before flipping their receiver females to flower.
Feminized pollen storage however is exactly the same process as regular pollen storage.

Fresh Pollen VS Stored Pollen

Although it is possible to store pollen for a long time, these tiny spores are very sensitive to temperature variations and humidity. The pollination may work very well but it can also fail. Pollinating your cannabis plants using fresh pollen is a sure-fire way to get lots of seeds.

6 thoughts on “Collecting And Storing Pollen. Cannabis Males & Reversed Females”

  1. Many wonder why reverse? Will the pollen taken and put on a viable female create some phenomenal plant? Will it single out it’s dominate genetic ancestor in the next grow? Will it increase THC? I reversed two male plants last year and .noticed lots of pollen and female pistols but only discovered small seeds hidden in some buds late in the season ..The trial and error of mistakes is not really mistakes and some of my females in the distance got some seed from the reverse pollen.. so I can only watch and wait for the visuals and terpenes

    1. Hi Roger,

      Sometimes breeding “Mistakes” can lead to some interesting discoveries!
      Thank you for sharing,

      The Khalifa team

    1. Thanks a lot Atom!!
      Usually, you can see a little bit of “powder” on the male flowers, that’s your pollen.
      Looking at your reversed plants in a dark room with a headlamp is the easiest way to know.

      Cheers,
      The Khlifa team

  2. So what does the mature male flower on a feminized plant look like? Do you wait till it’s dead? How do you collect the pollen without all of it sticking in the resin? I’m having problems, ugh. Feminized a few buds, have flowers, but they just look like they’re dying. 🙁

    1. Hey Shelly,

      Not all female plants can be successfully reversed. Sometimes they produce male flowers devoid of feminized pollen.
      Either you over-sprayed the plant or it is simply not the right candidate.

      Cheers,
      The Khalifa team

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